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Biology :: CRISPR :: What is the CRISPR CAS9 System?
 CRISPR technology is a simple yet powerful tool for editing genomes. It allows researchers to easily alter DNA sequences and modify gene function.

Its many potential applications include correcting genetic defects, treating and preventing the spread of diseases and improving crops. However, its promise also raises ethical concerns.

In popular usage, "CRISPR" (pronounced "crisper") is shorthand for "CRISPR-Cas9." CRISPRs are specialized stretches of DNA.

Intrigued? Learn more about this Genome Editing Tool here..
 CRISPRs: "CRISPR" stands for "clusters of regularly interspaced short palindromic repeats." It is a specialized region of DNA with two distinct characteristics: the presence of nucleotide repeats and spacers. Repeated sequences of nucleotides — the building blocks of DNA — are distributed throughout a CRISPR region. Spacers are bits of DNA that are interspersed among these repeated sequences. In the case of bacteria, the spacers are taken from viruses that previously attacked the organism. They serve as a bank of memories, which enables bacteria to recognize the viruses and fight off future attacks.

This was first demonstrated experimentally by Rodolphe Barrangou and a team of researchers at Danisco, a food ingredients company. In a 2007 paper published in the journal Science, the researchers used Streptococcus thermophilus bacteria, which are commonly found in yogurt and other dairy cultures, as their model. They observed that after a virus attack, new spacers were incorporated into the CRISPR region. Moreover, the DNA sequence of these spacers was identical to parts of the virus genome.

They also manipulated the spacers by taking them out or putting in new viral DNA sequences. In this way, they were able to alter the bacteria's resistance to an attack by a specific virus. Thus, the researchers confirmed that CRISPRs play a role in regulating bacterial immunity.


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keentween

  • Ask
  • FAQ
  • R Winter Series
    • Create & Manipulate Matrices
    • Data Cleaning, Data Frames & Lists
    • Tidyverse
    • Creating Graphs with ggplot
  • AP CS A
    • Java July Series >
      • :: Classes & OOPs
      • :: Interface
      • :: Inheritance
      • :: Intro To Arrays Java
      • :: Arrays Continued
    • :: CS-A or CS-P?
    • :: Control Structures
    • :: Recursion
    • :: While Do While Loops in Java
    • :: Interface in Java
    • :: ArrayLists
    • :: Java Naming Conventions
    • :: Logic Circuits
    • :: Getters and Setters
    • :: Binary & Hexadecimal
  • Summer 2020 Tutoring
    • :: REPRESENT IT!
    • Pre-Algebra Sessions >
      • :: Basic Division
      • :: Complex Division
      • :: Estimation Division
      • :: Division Practice Problems
    • Algebra II >
      • :: Cubic Equations
      • :: Complex Numbers
    • Chemistry >
      • Molarity Basics
    • C++ Sessions >
      • :: Introduction
      • :: Style v Syntax
      • :: Variables & Data Types
      • :: Intialize/Declare Variables
      • :: Types of Operators
      • :: Strings and Input - Output
      • :: How to Construct Arrays
  • AP Bio
    • :: Sketch Notes >
      • :: Part 1
      • :: Part 2
    • :: epigenetics
    • :: Chi-Squared Tests
    • :: Cancer
    • :: Hox Genes
    • :: Hardy-Weinberg Principle
    • :: Rule of Multiplication + Addition for Punnett Squares
    • :: CRISPR
    • :: Amino Acid
    • :: Peptide
    • :: Why study Peptides
    • :: Aquaporins
    • :: Gram Stains
    • :: Graph on Excel for Bio Lab
  • AP Chem
    • Organic Chemistry
    • I. Properties of Matter >
      • Neutralization
    • II. Periodic table >
      • Org of Periodic Table
      • :: Groups
    • III. Chemical bonding >
      • :: Mass to Mass conversion
      • :: Naming Acids
      • :: Cross Drop Charge
      • :: Predicting Products
      • :: Balance Equation Question
      • :: Learn to Balance Equation
      • :: VSEPR Simulation
    • IV. Molar Mass >
      • ::LR ER and excess reatant
      • :: Molecular/Formula Mass
      • :: Empirical Formula & Molar Mass
      • :: Percentages & Empirical Formula
      • :: Empirical formula
    • IV. Solutions and Solubility >
      • :: Types of Solutions with Solubility Curves
      • :: Solubility Curve
    • V. Easy Tricks and Tips >
      • :: Tip to Molecular Shapes
      • Memorizing Bond Angles and Polarity
      • :: Chemistry Formulas
      • :: Trick Polyatomic ions
    • VI. General AP Concepts >
      • :: Potential Energy Diagrams
      • :: Haber-Bosch
      • :: Le Chatelier
      • :: Pressure & Moles
      • ::Rydberg's Constant vs Unit of Energy
      • :: Equilibrium and RICE Tables
      • :: Kinetics
      • Galvanic Cells
    • :: Flash cards
    • :: VSEPR
  • AP Stats
    • Chi-Squared Tests
    • Solving Chi-Sqd Test Using Sheets
    • Applications of Statistics
    • Standardized Scores
    • Distributions Transformations
  • AP Calc
    • DI Method - Tabular Integration
    • Polar Curves: Tangent Line and Slope
    • Riemann Sums: Left and Right Approximations
    • :: Conic Sections Flash cards
    • :: Parent Functions Flash cards
    • Worked Out Problems >
      • :: Worked Out Problems I
      • :: Worked Out Problems II
      • :: Worked Out Problems III
      • :: Worked Out Problems IV
      • :: Worked Out Problems V
      • :: Worked Out Problems VI
      • :: Worked Out Problems VII
      • :: Worked Out Problems VIII
      • :: Worked Out Problems IX
      • :: Worked Out Problems X
      • :: Worked Out Problems XI
      • :: Worked Out Problems XII
      • :: Worked Out Problems XIII
    • Applying Trig Identities
    • L'Hopital's Rule
    • Differences Between Conic Sections
    • Graphing Conic Sections
    • :: Pre-Calc - Trig Identities
    • Tangent & Normal Lines
    • Indefinite integrals: U Sub
    • Calculus Derivatives >
      • Product Rule
      • Quotient Rule
      • Chain Rule
  • Arduino
    • Quick Look
    • Project #1: Blinking LED
    • Project #2: Button LED
    • Project #3: Flowing LED
    • Project #4: LCD Display
    • Project #5: Serial Monitor
  • AP Español
    • AP Español Salsa
  • App
    • AP Go Pow How?
    • AP Go Pow APP Page
  • Musings
    • :: Bayesian Example
    • :: Nash equilibria
    • :: Bayesian Nash Equilibrium
    • :: Backward induction
    • :: what is ISS
    • :: Rotational Matrices
    • :: Primary v Secondary Pollutants
    • :: Black Hole
    • :: Covid-19 Hackathon
    • :: Evolution of Immunizations
    • :: Predictions of Diseases
    • :: Book List
    • :: Patterncount
    • :: Binary Classification
    • :: Cybersecurity
    • :: What is CIA Triad
    • :: What is Networking
    • :: Self Similarity
    • :: Trig Identities
    • :: UIL Number S
    • :: Box Offensive Play
    • :: Why Card Trick Works
    • :: Easy Multiplication
  • AP CREDIT
  • About